De Novo Protein Attachment to Hemagglutinin

Model of Hemagglutinin (rose) with De Novo Binding Protein Bound (blue)(Derived from 3R2X.pdb)

The Two Subunits of Hemagglutinin

HA1 (Chain 1): Blanched Almond
HA2 (Chain 2): Red, Yellow, Blue, Light Blue

• A Helix (residues 38-58): Red
  
• CD Helix (residues 75-126): Yellow
  
• Connecting Loop (residues 59-74): Blue
  
• Remainder of Subunit (residues 1-37, 127-173): Light Sky Blue
  

Important Residues on Hemagglutinin

Important Residues on HA that interact with HCDR1 Residues Pro28, Phe29, Phe74

• HA1 Residues Val40, Leu42, Leu292: CPK
  
• HA2 Residues Thr49, Val52, Ile56: CPK
  

Important Residues on HA that interact with conserved hydrophobic tip of HCDR1 residues Ile53, Phe54, Tyr98

• HA1 Residues His18, His38: CPK
  
• HA2 Residues Trp21, Thr41, Ile45: CPK
  

Backbone Model of De Novo Binding Protein HB36.3 and Its Important Residues

• Protein backbone: orchid
  
• Hydrophobic residues (in the HA"pocket") Phe61,Trp57,Met53,Phe49: CPK
  

Spacefilled Model of De Novo Binding Protein HB36.3 and Its Important Residues

• Protein backbone: orchid
  
• Hydrophobic residues (in the HA"pocket") Phe61,Trp57,Met53,Phe49: CPK
  

Spacefill Model of Antibody CR6261 attached to HA

Additional images showing the binding between hemagglutinin and the De Novo binding Protein HB36.3